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Microbiology Objective Questions { Physical and Chemical Agents }

36. Which of the following is a limitation of the autoclave?
A.It takes too long to sterilize
B.It lacks the ability to inactivate viruses
C.It lacks the ability to kill endospores
D.It will destroy heat labile materials
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37. Which of the following has most heat resistant spores?
A.Clostridium histolyticum
B.Clostridium perfringens
C.Bacillus stearothermophilus
D.Bacillus cereus
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38. The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the
A.decimal reduction time
B.thermal death point
C.thermal death temperature
D.thermal death time
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39. Which of the following tests is designed to simulate the natural conditions under which the disinfectants are used in hospitals?
A.Phenol coefficient test
B.Minimum inhibitory concentration test
C.Capacity test
D.None of these
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40. Steam exposure of a material at 100°C for 20 minutes for three consecutive days is known as
D.none of these
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41. Which of the following material is sterilized by use of red heat?
A.Soiled dressings
B.Inoculating wires and loops
C.Glass slides
D.All-glass syringes
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42. Which of the following methods will not sterilize?
A.Aqueous glutaraldehyde for 10 hours
B.Dry heat at 171°C for 1 to 2 hours
C.121°C at 15 lb/in pressure for 15 to 20 minutes
D.100°C boiling water for 30 minutes
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