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Biotechnology Objective Questions { Vectors Uses for Animal Cell Culture }



8. In case of Baculoviruses, the foreign gene
A.is inserted downstream of polyhedrin promoter
B.is inserted downstream of viral origin of replication
C.is inserted between the polyhedrin promoter and downstream sequences
D.is inserted randomly in viral genome
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9. α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is
A.His 3
B.Lys 2
C.Ura 3
D.Trp 1
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10. Yeast episomal plasmids (YEp) were constructed using
A.2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
B.2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
C.12μm yeast plasmid and E coli vector pMB 9
D.12μm yeast plasmid and E coli vector pBR 322
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11. Which of the following is/are commonly used promoter of S. cerevisiae?
A.Galactokinase (GAL 1)
B.Alcohol dehydrogenase II (ADH 2)
C.Phosphoglycerate kinase (PGK)
D.All of the above
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12. Maximum amount of foreign DNA introduced into SV 40 viruses is
A.10 kb
B.5 kb
C.2.5 kb
D.1 kb
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13. Recombinant SV40 viruses introduce the foreign DNA into cells
A.with the DNA mediated transfection
B.without the DNA mediated transfection
C.with the RNA mediated transfection
D.without the RNA mediated transfection
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14. Which one of the following is the most stable vector?
A.YEp
B.YRp
C.YAC
D.YIp
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