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Biotechnology Objective Questions { Plant Cell Culture }

1. A(n) __________ is an excised piece of leaf or stem tissue used in micropropagation.
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2.  Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with
A.cellulotyic enzymes
B.pectolytic enzymes
C.both cellulotyic and pectolytic enzymes
D.proteolytic enzymes
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3. Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?
A.Multiplication of sexually derived sterile hybrids
B.Less multiplication of disease free plants
C.Storage and transportation of propogates
D.Both (b) and (c)
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4. What is meant by 'Organ culture' ?
A.Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
B.Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
C.Cultivation of organs in a laboratory through the synthesis of tissues
D.The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
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5. Which method of plant propagation involves the use of girdling?
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6. Organogenesis is
A.formation of callus tissue
B.formation of root and shoots on callus tissue
C.both (a) and (b)
D.genesis of organs
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7. Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?
A.Nurse medium
B.Nurse or feeder culture
C.Both (a) and (b)
D.None of these
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