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Biotechnology Objective Questions
{ Gene Transfer in Plants }
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Biotechnology
// Gene Transfer in Plants
1. Virulence trait of Agrobacterium tumefaciens is borne on
A. chromosomal DNA
B. tumour inducing plasmid DNA
C. both chromosomal and plasmid DNA
D. cryptic plasmid DNA
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2. The size of the virulent plasmid of Agrobacterium tumefaciens is
A. 40-80 kb
B. 80-120 kb
C. 140-235 kb
D. >235 kb
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3. Which of the following is not true about the helper plasmids?
A. These can replicate in Agrobacterium
B. These help in the mediating conjugation of intermediate vectors
C. These can't replicate in Agrobacterium
D. All of the above
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4. Which technique is used to introduce genes into dicots?
A. electroporation
B. particle acceleration
C. microinjection
D. Ti plasmid infection
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5. Direct DNA uptake by protoplasts can be stimulated by
A. polyethylene glycol (PEG)
B. decanal
C. luciferin
D. all of these
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6. In a plant tumour cell
A. complete Ti plasmid is incorporated in plant nuclear DNA
B. different parts of the Ti-plasmid are incorporated
C. only a small specific segment of callus T DNA is incorporated
D. may vary from plant to plant
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7. Co-integrating transformation vectors must include a region of homology in
A. the vector plasmid
B. the Ti-plasmid
C. between vector plasmid and Ti-plasmid
D. none of these
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