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Biotechnology Objective Questions { Cloning Vectors }



15. P1 cloning vector is the example of
A. plasmid
B. cosmid
C. bacteriophage
D. phagemid
Discussion
16. Cos site of the cosmids
A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. both (a) and (b)
D. contains cleavage site
Discussion
17. M 13 is an example of
A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid
Discussion
18. Phagemid vectors are
A. combination of plasmid and phage λ
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. all of the above
Discussion
19. Single stranded vectors are useful
A. for sequencing of cloned DNA
B. for oligonucleotide directed mutagenesis
C. for probe preparation
D. all of the above
Discussion
20. Inserted DNA in λ gt 11 can be expressed as
A. β-galactosidase fused protein
B. free protein in the cytoplasm
C. free protein that is secreted out
D. all of the above
Discussion
21. EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
A. 6-7 kb
B. 15-25 kb
C. 40-44 kb
D. 1-2 kb
Discussion

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