1. Maximum size of foreign DNA that can be inserted into a replacement vector is
A.25-30 kb
B.18-20 kb
C.20-25 kb
D.40-50 kb

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2. Which of the following is not true about phagemid?
A.Contain functional origin of replication of the plasmid and λ phage
B.May be propagated as a plasmid or as phage in appropriate strain
C.Contain λ att site
D.Can only be propagated as phage

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3. pBR 322 has/have which of the following selection marker(s)?
A.Ampr
B.Tetr
C.Both (a) and (b)
D.Kanr

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4. A plasmid can be considered as a suitable cloning vector if
A.it can be readily isolated from the cells
B.it possesses a single restriction site for one or more restriction enzymes
C.insertion of foreign DNA does not alter its replication properties
D.All of the above

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5. Difference between λ gt 10 and λ gt 11 vectors is that
A.λ gt 11 is an expression vector
B.λ gt 10 is an expression vector
C.λ gt 10 is a replacement vector
D.λ gt 11 is a replacement vector

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6. λ ZAP vector is an example of
A.phage
B.phagemid
C.cosmid
D.plasmid

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7. λ gt 10 and λ gt 11 vectors can propagate cloned fragments up to
A.6-7 kb
B.1-2 kb
C.40-44 kb
D.20-23 kb

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