Recombinant DNA Technology MCQs

Biochemistry Objective Questions

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22. An example of a restriction fragment length polymorphism is

A. an Eco RI cuts DNA at a different sequence than Hind III

B. different length fragments of DNA resulting from loss or gain of a restriction site

C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

D. all of the above

23. Knockout mice are created by

A. mutagenizing a mouse and selecting for mutant offspring

B. creating a chimera by fusing cells from two different cell lines

C. infecting the mouse with a retrovirus

D. transfecting embryonic stem cells with an altered gene sequence

24. A plasmid

A. is a circular DNA molecule

B. always contains an origin of replication

C. usually contains one or more restriction sites

D. all of the above

25. Under which of the following conditions would population gene frequencies remain the same?

A. Selection for homozygotes

B. Small population size

C. Active migration between groups

D. Random mating

26. Plant transformation can be obtained by

A. combining plant and animal cells in culture

B. shooting DNA into plant cells with a gun

C. using the E. coli bacterium to infect plant roots

D. infecting plants with a tobacco mosaic virus

27. An expression vector

A. always contains an origin of replication

B. usually contains a gene that confers antibiotic resistance to the bacterial host

C. always contains DNA segments for the regulation of mRNA production

D. all of the above

28. The unpaired nucleotides produced by the action of restriction enzymes are referred to have

B. single strands

C. restriction fragments

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