DNA Structure and Replication MCQs

Biochemistry Objective Questions

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92. During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of

A. high temperature and Ca++

B. low temperature and Ca++

C. high temperature and Mg++

D. low temperature and Mg++

93. What is the exact name of the classical Watson-Crick double helix DNA?

94. The site on a bacterial chromosome that marks the initiation point for chromosome replication is called

D. an initiation codon

95. The B-DNA structure found in solution is a

A. left-handed double helix of antiparallel chains (10 bp/turn)

B. right-handed double helix of antiparallel chains (∼10 bp/turn)

C. left-handed zig-zag helix of antiparallel chains (10 bp/turn)

D. right-handed double helix of parallel chains (∼10 bp/turn)

96. Which will require a higher temperature to denature?

A. DNA with a high G-C content

B. DNA with a high A-T content

C. RNA with a high G-C content

D. RNA with a high A-T content

97. In an agarose gel, DNA is moving from

A. cathode to anode

B. left to right

C. anode to cathode

98. TM refers to the temperature at which

A. membranes are 50% fluid

B. 50% of a DNA molecule is denatured

C. 50% of a protein molecules are denatured

D. all of the above

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