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Biochemical Engineering Objective Questions { Immobilized Enzyme }



15. Commonly employed water insoluble supports for the covalent attachment of enzyme include
A.acrylamide based polymers
B.polypeptides
C.dextran
D.all of these
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16. Which of the following is not a physical method of immobilization?
A.Adsorption
B.Entrapment
C.Micro encapsulation
D.None of these
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17.  The support material for immobilization of cells of Bacillus subtilis is
A.ion exchange resins
B.gelatin
C.Anthracite
D.agarose and carbodiimide
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18. The intraparticle mass transfer resistance can affect the rate of enzyme reaction, if enzymes are immobilized by
A.copolymerization or microencapsulation
B.crosslinking using multifunctional reagents
C.adsorption
D.all of the above
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19. The covalent attachment of enzyme molecules is via
A.nonessential amino acids residues to water insoluble, functional supports
B.essential amino acids residues to water insoluble, functional supports
C.nonessential amino acids residues to water soluble, functional supports
D.essential amino acids residues to water soluble, functional supports
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20.  To measure the extent to which the reaction rate is lowered because of resistance to mass transfer, the effectiveness factor of an immobilized enzyme, η can be defined as
A.reaction rate/rate if not slowed by diffusion
B.rate if not slowed by diffusion/ reaction rate
C.actual reaction rate/ rate if not slowed by diffusion
D.rate if not slowed by diffusion/ actual reaction rate
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21. Which is not the method for producing immobilized enzymes with multifunctional reagents?
A.Enzymes are adsorbed on the surface active support followed by intermolecular cross linking
B.Functional groups are introduced on the support to react co-valently with enzymes
C.Enzymes are cross linked intermolecularly
D.None of the above
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