Biochemical Engineering Objective Questions { Immobilized Enzyme }

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1. Water insoluble enzymes can be prepared by using multifunctional agents that are bifunctional in nature and have

A. low molecular weight

B. high molecular weight

C. high equivalent weight

D. low reactivity

2. Functional groups of the nonessential amino acid residues that are suitable for the immobilization process are

A. free α-, β- or γ carb oxyl groups

B. α or β amino groups

C. phenyl, hydroxyl, sulfhydryl or imidazole groups

D. all of the above

3. Which medium is used for the production of Penicillin using immobilized cells

A. 1% peptone medium

B. glucose medium

C. Yeast extract medium

4. Which of the following is taken as an assumption in the distributed model?

A. The reaction occurs at every position and the kinetics of the reaction are of the same form as observed for free enzyme.

B. Mass transfer through the immobilized enzyme occurs via molecular diffusion

C. There is no mass transfer limitation at the outside surface of the spherical immobilized enzyme

D. All of the above

5. The immobilized enzyme produced by micro encapsulation technique provides

A. an extremely large surface area

B. smaller surface area

C. high amount of solvent

D. relatively smaller surface area

6. Which of the following is considered as a disadvantage to the adsorption method of immobilization?

A. It is possible to separate and purify the enzymes while being immobilized

B. The enzymes are not usually deactivated by adsorption

C. The adsorption is a reversible process

D. State of immobilization is very sensitive to solution pH, ionic strength and temperature

7. During the enzymatic reaction of an immobilized enzyme, the rate of substrate transfer is

A. equal to that of substrate consumption

B. more than that of substrate consumption

C. lesser than that of substrate consumption

D. is nothing to do with the substrate consumption

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